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21/12/2016
 

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 (Trifolium pratense \ red clover .

.

, , .

, -.

, , , / .

.

 

,  2004  .

-205   49-65, (Wolfe P2\DY), . (26 " Biochanin A, 16 " Formononetin, 1 " Genistein 0.5 " Daidzein) . .

, (1).

 

 2005, (Pueraria lobata), . -25  ,  12 , .

-46%  :  9.7  -5.2.

, (2).

 

,  2008   40 " . .

-407   35-70, , -3 . . (3).

 

 2004  , () .

, , (4).

 

, -2008   Biochanin A, , (Aromatase),  MCF-7. -Biochanin A  , . (5).

 

 2012  -  Biochanin A, (Ester, Carbamate-Ester), .

. -Biochanin A, -. , (6).

 

(Formononetin).

 2011   MCF-7 ( ). -, , -.

, . -  mRNA  - (7).

 

 2012, , - (8).

 

 2014  .

, .

, , - (MMP-2, MMP-9) (PI3K/AKT). – - (9).

 

 2011,  11  , (, , ). , , (10).

 

 2014  .

- . . -, : > > Biochanin A> > .

, . . , , (11).

 

.

-,  2002  . -38  , 20   160 " .  PSA, .

, ,  1-3  . , (12).

 

 2011  -Biochanin A  -  TRAIL, (LNCaP -du145). -Bichanin A  . TRAIL -  NFk-B  (13) -TRAIL.

 

 2012  Formononetin  .

,  LNCaP ( )(14).

 

 2014  -Formononetin  (PC-3) .

 PC-3, -, .

, (15).

Bax-Bcl-2   P38\Akt.

 

-Formononetin   DU-145  .

 Formononetin  ,  Bcl-2 -Bax.

-. (16) (  Bcl-2   Bax -rasd1).

 

, .

, , . 

,     , , ().

 

:

  1. Atkinson C, Warren RM, Sala E, Dowsett M, Dunning AM, Healey CS, Runswick S, Day NE, Bingham SA. Red-clover-derived isoflavones and mammographic breast density: a double-blind, randomized, placebo-controlled trial [ISRCTN42940165]. Breast Cancer Res. 2004;6(3):R170-9. 
  2. Lukaczer D, Darland G, Tripp M, Liska D, Lerman RH, Schiltz B, Bland JS. Clinical effects of a proprietary combination isoflavone nutritional supplement in menopausal women: a pilot trial. Altern Ther Health Med. 2005 Sep-Oct;11(5):60-5.
  3. Powles TJ, Howell A, Evans DG, McCloskey EV, Ashley S, Greenhalgh R, Affen J, Flook LA, Tidy A. red clover isoflavones are safe and well tolerated in women with a family history of breast cancer. Menopause Int. 2008 Mar;14(1):6-12. doi: 10.1258/mi.2007.007033.
  4. Bodinet C, Freudenstein J. Influence of marketed herbal menopause preparations on MCF-7 cell proliferation. Menopause. 2004 May-Jun;11(3):281-9.
  5. Wang Y, Man Gho W, Chan FL, Chen S, Leung LK. The red clover (Trifolium pratense) isoflavone biochanin A inhibits aromatase activity and expression. Br J Nutr. 2008 Feb;99(2):303-10. Epub 2007 Aug 29.
  6. Fokialakis N, Alexi X, Aligiannis N, Siriani D, Meligova AK, Pratsinis H, Mitakou S, Alexis MN. Ester and carbamate ester derivatives of Biochanin A: synthesis and in vitro evaluation of estrogenic and antiproliferative activities. Bioorg Med Chem. 2012 May 1;20(9):2962-70. doi: 10.1016/j.bmc.2012.03.012. Epub 2012 Mar 10.
  7. Chen J, Zeng J, Xin M, Huang W, Chen X. Formononetin induces cell cycle arrest of human breast cancer cells via IGF1/PI3K/Akt pathways in vitro and in vivo. Horm Metab Res. 2011 Sep;43(10):681-6. doi: 10.1055/s-0031-1286306. Epub 2011 Sep 19.
  8. Chen J, Sun L. Formononetin-induced apoptosis by activation of Ras/p38 mitogen-activated protein kinase in estrogen receptor-positive human breast cancer cells. Horm Metab Res. 2012 Dec;44(13):943-8. doi: 10.1055/s-0032-1321818. Epub 2012 Jul 24.
  9. Zhou R, Xu L, Ye M, Liao M, Du H, Chen H. Formononetin inhibits migration and invasion of MDA-MB-231 and 4T1 breast cancer cells by suppressing MMP-2 and MMP-9 through PI3K/AKT signaling pathways. Horm Metab Res. 2014 Oct;46(11):753-60. doi: 10.1055/s-0034-1376977. Epub 2014 Jun 30.
  10. Reiter E, Gerster P, Jungbauer A. red clover and soy isoflavones--an in vitro safety assessment. Gynecol Endocrinol. 2011 Dec;27(12):1037-42. doi: 10.3109/09513590.2011.588743. Epub 2011 Aug 1.
  11. Spagnuolo P, Rasini E, Luini A, Legnaro M, Luzzani M, Casareto E, Carreri M, Paracchini S, Marino F, Cosentino M. Isoflavone content and estrogenic activity of different batches of red clover (Trifolium pratense L.) extracts: an in vitro study in MCF-7 cells. Fitoterapia. 2014 Apr;94:62-9. doi: 10.1016/j.fitote.2014.01.027. Epub 2014 Feb 5.
  12. Jarred RA, Keikha M, Dowling C, McPherson SJ, Clare AM, Husband AJ, Pedersen JS, Frydenberg M, Risbridger GP. Induction of apoptosis in low to moderate-grade human prostate carcinoma by red clover-derived dietary isoflavones. Cancer Epidemiol Biomarkers Prev. 2002 Dec;11(12):1689-96.
  13. Szliszka E, Czuba ZP, Mertas A, Paradysz A, Krol W. The dietary isoflavone biochanin-A sensitizes prostate cancer cells to TRAIL-induced apoptosis. Urol Oncol. 2013 Apr;31(3):331-42. doi: 10.1016/j.urolonc.2011.01.019. Epub 2011 Jul 30.
  14. Ye Y, Hou R, Chen J, Mo L, Zhang J, Huang Y, Mo Z. Formononetin-induced apoptosis of human prostate cancer cells through ERK1/2 mitogen-activated protein kinase inactivation. Horm Metab Res. 2012 Apr;44(4):263-7. doi: 10.1055/s-0032-1301922. Epub 2012 Feb 10.
  15. Zhang X, Bi L, Ye Y, Chen J. Formononetin induces apoptosis in PC-3 prostate cancer cells through enhancing the Bax/Bcl-2 ratios and regulating the p38/Akt pathway. Nutr Cancer. 2014;66(4):656-61. doi: 10.1080/01635581.2014.894098. Epub 2014 Mar 25.
  16. Liu XJ, Li YQ, Chen QY, Xiao SJ, Zeng SE. Up-regulating of RASD1 and apoptosis of DU-145 human prostate cancer cells induced by formononetin in vitro. Asian Pac J Cancer Prev. 2014;15(6):2835-9.